Electron Microscopy Department

Electron microcopy department includes 3 main laboratories:

  • 1- Transmission Electron Microscopy (TEM)
  • 2- Scanning Electron Microscopy (SEM)
  • 3- Sample Preparation Laboratory

  • Electron Microscopy Departmenty is equipped with the leading company for Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM), ThemroFisher Scientific (formerly FEI), Talos for TEM and Quattro for SEM. Sample preparation laboratory for electron microscopy is equipment with RMC (USA) and Leica Microsystems.

    • Transmission Electron Microscopy (TEM) is a technique in which high energy electrons are transmitted through ultrathin sample (~100nm thickness). These thin samples interact with the electrons as it passes through it and images are formed.
    • Scanning Transmission Electron Microscopy (STEM) is a similar method to TEM but STEM is distinguished from TEM by focusing the electron beam into a narrow spot which is scanned over the sample in a raster. By using TEM or STEM, a high-quality image could be generated at atomic scale resolution which is around 3-4Å. The images are applied in clinical diagnostic, cancer research, virology, material science and other basic research.
    • Cryo-Transmission Electron Microscopy (Cryo-TEM) is a powerful tool for macromolecular structural determination in the entry level of biological and material sciences research. The specimen is maintained in a near-native environment (without chemical treatment) within a thin film of vitreous ice and imaged in a transmission electron microscope (TEM).
    • Electron Tomography (ET) is a Three-dimensional (3D) structural analysis that is essential to understand the relationship between the structure and function of an object. ET retrieves 3D structural information from a tilt series of 2D projections, and is gradually becoming a mature technology with sub-nanometer resolution. This is one of the most popular analytic tool and has been widely used for structural analysis in both physical and biological sciences.
    • ExpressMed laboratories offer above services with high quality and excellent technical and professional support. Our customer services team is available to help to achieve your specific research goals.

    Technical Specifications

    • Thermo scientific(FEI) Talos L120C G2
    • TEM operating voltage 20-120kV
    • Beam source: LaB6 filament.
    • TEM line resolution ≤ 0.204 nm
    • TEM point resolution ≤ 0.37 nm
    • STEM HAADF resolution with LaB6 (nm) ≤ 1.0 nm
    • High-quality imaging, 4k × 4k Ceta CMOS camera provides a large field-of-view and live digital zooming with high sensitivity and high speed

    Imaging Techniques

    • Transmission Electron Microcopy (TEM)
    • Scanning Transmission Electron Microscopy (STEM)
    • Cryo-Transmission Electron Microscopy (Cryo-TEM)
    • 2D and 3D Electron Tomography

    Clinical application:

    • Pathology specimens (Renal biopsies, muscle biopsies, and nerve biopsies)
    • Cilia
    • Liver
    • Cancer
    • Infectious/virology

    Research application:

    • Biology with its different branches including:
    • Cell biology and biophysics
    • Developmental biology
    • Genome biology
    • Structural and computational biology

    EM Renal Biopsy
    Capillary loop
    EM Renal Biopsy
    Electron dense deposits
    EM Renal Biopsy
    Electron dense deposits
    EM Renal Biopsy
    GBM measurments

    • Nano particles characterization, size, core/shell investigation.
    • Defect analysis
    • Colloidal dispersion of liposomes, polymerase, emulsion
    • Soot particle analysis
    • Catalyst study

    Gold nano partilce morphology on carbon film
    High resolution image graphitized carbon lattice plane spacing showing 0.37 nm

    Cutting Edge Sample Preparation Lab Equipped RMC Boeckeler Instruments, USA

    • Produce high quality semi- and ultra-thin sections for TEM, SEM..
    • Specifications: Specimen thickness range from 5nm to15 µm.

    Cryo Ultramicrotome- LN Ultra

    Sectioning of both biological and industrial materials can be achieved with the LNUltra, a liquid nitrogen-cooled cryochamber attachment for the PowerTome series of ultramicrotome. Mounted onto the PowerTome, the LN Ultra provides optimal thermal stability necessary for producing cryosections. Capable of holding temperatures from +40°C to -185°C, the stable environment of the LNUltra is perfect for sectioning and planing a variety of samples. These may include rubber and soft polymers, or biological cells and tissues; used for example, for immunolabelling using the Tokuyasu technique or CEMOVIS (Cryo Electron Microscopy of Vitreous Ice Sections).

    Diamond Knife for Ultra-Thin Sections

    Automated EM Tissue Processor

    For tissue processing and resin embedding, automation is the safest and most reproducible route, minimize cross contaminations.

    Automated EM Stainer

    After Ultramicrotomy, most sections require contrasting with lead and Uranyl salts. QG3100, capable of contrasting up to 40 grids in one run.

    Gold nano partilce morphology on carbon film
    High resolution image graphitized carbon lattice plane spacing showing 0.37 nm
    Diamond knives for Ultramicrotomy
    Automated EM Tissue Processor
    Automated EM Stainer

    Timely TEM service
    We can turn around your TEM sample in a day or two with priority support, or about one week with normal support. Either way you get numerous crisp & clear TEM images and a brief, but comprehensive analysis report. That’s responsive service.

    Saving you money
    We keep our overhead costs low, so we can pass the savings on to you. We offer great introductory rates and volume discounts that can save you a significant amount of money. We help you stretch your budget dollars.

    Win/Win relationships
    We want to establish and maintain long-term, mutually beneficial relationships with our clients. This will insure your success, as well as ours. Our clients’ continuing success is the foundation of our company.

    Helping you hit your targets
    We are committed to helping you to meet your targets. Whether that means helping you enhance your yield so you can hit your production targets, or by helping you further your research endeavors. We will be there by your side, working with you.

    Need more info? Don't hesitate!

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